Dr. Carsten Sanders

Dr. Carsten Sanders Photo
Contact Information

Carsten Sanders, Ph.D.
Assistant Professor of Chemistry
Department of Physical Sciences
Kutztown University of Pennsylvania
Boehm Science Building Room 116
PO Box 730 | Kutztown, PA 19530
Phone: 484-646-5862
Fax: 610-683-1352

Courses taught at Kutztown University

Physical Sciences Department (starting in Fall 2014)

FYS100 First-Year Seminar – Microbes, Humans, Epidemics1

CHM100 General Chemistry I1+2

CHM102 General Chemistry II1+2

CHM204 Fundamentals of Organic Chemistry1+2

CHM214 Organic Chemistry I2

CHM216 Organic Chemistry II2

CHM310 Biochemistry I2

CHM312 Biochemistry II1+2

CHM318 Advanced Biochemistry1

CHM370-373 Research in Chemistry I-IV3

Biological Sciences Department (from Spring 2009 till Spring 2014)

BIO010 Biology for Nonmajors1+2

BIO104 Principles of Biology2

BIO161 Introduction to Microbiology2

BIO216 Genetics2

BIO224 Applied Environmental Microbiology1+2

BIO336 Medical Microbiology1+2

BIO370 Research in Biology I3

(1: Lecture; 2: Laboratory; 3: Independent Study)

Current Research Projects

We are currently working on different aspects related to the biogenesis c-type cytochromes. These are proteins that act as electron carriers between respiratory or photosynthetic energy transduction pathways, but are also part of catalytically active protein complexes and are involved in apoptosis (programmed cell death). Each functional cytochrome c (called holocytochrome c) contains at least one heme b (protoporphyrin IX-Fe) cofactor bound covalently to a precursor polypeptide chain (called apocytochrome c) via (usually) two thioether bonds between the vinyl groups of the heme-porphyrin ring and the cysteine-sulfhydryl groups of a conserved Cys-Xxx-Xxx-Cys-His heme-binding site within the apocytochrome c [1-3]. Multiple distinct biogenesis machineries responsible for the post-translational attachment of heme b compounds to apocytochromes c have been identified in various organisms and organelles [4-7]. Focusing on the biogenesis machinery that is confined to mitochondria of fungi, metazoans and some protozoa (called Ccm system III), we try to determine on the molecular level how the catalytic component of this biogenesis machinery (called cytochrome c heme lyase or holocytochrome c synthetase) binds its substrates (heme b or apocytochrome c) and how this component performs the thioether bond formation between them.

References:

[1] G.W. Pettigrew, G.R. Moore, Cytochrome c: Biological Aspects, Springer Verlag, Berlin,   1987.

[2] S.E. Bowman, K.L. Bren, The chemistry and biochemistry of heme c: functional bases for covalent attachment, Nat Prod Rep 25 (2008) 1118-1130.

[3] C. Sanders, S. Turkarslan, O. Onder, E. R. Frawley, R. G. Kranz, H. G. Koch & F. Daldal, Biogenesis of c-type cytochromes and cytochrome complexes. The Purple Phototrophic Bacteria (Chapter 21), Volume 28 of Advances in Photosynthesis and Respiration, edited by Hunter et al., Dordrecht (NL): Springer Verlag, pp. 407-423.

[6] C. Sanders, S. Turkarslan, D.W. Lee, F. Daldal, Cytochrome c biogenesis: the Ccm system, Trends Microbiol 18 (2010) 266-274.

[7] J.W. Allen, Cytochrome c biogenesis in mitochondria - Systems III and V, FEBS J 278 (2011) 4198-4216.

[8] J. Simon, L. Hederstedt, Composition and function of cytochrome c biogenesis System II, FEBS J 278 (2011) 4179-4188.

[9] C. de Vitry, Cytochrome c maturation system on the negative side of bioenergetic membranes: CCB or System IV, FEBS J 278 (2011) 4189-4197.

Recent Publications and Presentations (since 2009)

Peer-Reviewed Publications

Verissimo, Khalfaoui-Hassani, Hwang, Steimle, Selamoglu, Sanders, Khatchikian & Daldal (2017) The thioreduction component CcmG confers efficiency and the heme ligation component CcmH ensures stereo-specificity during cytochrome c maturation. J Biol Chem 292:13154-13167 (doi: 10.1074/jbc.M117.794586)

Verissimo, Sanders, Daldal & Sanders (2012) Engineering a prokaryotic apocytochrome c as an efficient substrate for Saccharomyces cerevisiae cytochrome c heme lyase. Biochem Biophys Res Commun 424: 130-135 (doi: 10.1016/j.bbrc.2012. 06.088)

Verissimo, Honghui, WU, Sanders & Daldal (2011) CcmI subunit of CcmFHI heme ligation complex functions as an apocytochrome c chaperone during c-type cytochrome maturation. J Biol Chem 286: 40452-40463 (doi: 10.1074/jbc.M111.277764)

Sanders, Turkarslan, Lee & Daldal (2010) Cytochrome c biogenesis: the Ccm system. Trends Microbiol 18: 266-274 (doi: 10.1016/j.tim.2010.03.006)

Im, Smith, Sanders, Melara, Taylor, Lech & Chance (2010) Effect of CoQ10 supplementation on oxidative stress, skeletal muscle blood flow and mitochondrial function in older adults. Med Sci Sport Exercise 42(5): p3 (doi: 10.1249/01.MSS.0000384236. 81545.d9)

Panavas, Sanders & Butt (2009) SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems. SUMO protocols (Chapter 20), Volume 497 of Methods in Molecular Biology, edited by Ulrich, pp. 303-317. Totowa (NJ, USA): Humana Press (doi: 10.1007/978-1-59745-566-4_20)

Sanders, Turkarslan*, Onder, Frawley, Kranz, Koch & Daldal (2009) Biogenesis of c-type cytochromes and cytochrome complexes. The Purple Phototrophic Bacteria (Chapter 21), Volume 28 of Advances in Photosynthesis and Respiration, edited by Hunter et al., pp. 407-423. Dordrecht (NL): Springer Verlag (doi: 10.1007/978-1-4020-8815-5_21)

Selected Professional Presentations at Scientific Meetings

Roberts*, Junker & Sanders (2019) Microphthalmia with Linear Skin Defects Syndrome (MLS) on the Molecular Level – Probing Cytochrome c Heme Lyase E133 and R199 Mutants. 47th Middle Atlantic Regional Meeting (MARM) of the American Chemical Society (ACS) in Baltimore (PA, USA), May 30-June 1

Munoz*, Salazar* & Sanders (2019) Alteration of Substrate Binding and Ligation by Cytochrome c Heme Lyase Mutants. 30th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 5

Burke*, Tran* & Sanders (2019) Heme Protein Fragments as Bioengineering Tools. 30th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 5

Carpenter*, Cannon*, & Sanders (2019) Plant c-type Cytochromes as Protein Substrates for Yeast Cytochrome c Heme Lyase. 30th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 5

Salazar*, Munoz* & Sanders (2018) Examining Functional Cytochrome c Formation by Cytochrome c Heme Lyase Mutants. 5thAnnual Pennsylvania State System of Higher Education (PASSHE) Undergraduate Research Conference in Science, Technology, Engineering and Mathematics (STEM) at Millersville University in Millersville (PA, USA), Oct. 27

Roberts*, Junker & Sanders (2018) Studying the Effects of Mutations of a Conserved Glutamic Acid Residue (E133) on the Activity of Cytochrome c Heme Lyase. 5thAnnual Pennsylvania State System of Higher Education (PASSHE) Undergraduate Research Conference in Science, Technology, Engineering and Mathematics (STEM) at Millersville University in Millersville (PA, USA), Oct. 27

Cannon*, Carpenter* & Sanders (2018) Testing the Ability of Yeast Cytochrome c Heme Lyase to use Cytochrome c Protein Substrates originated from Plants. 5thAnnual Pennsylvania State System of Higher Education (PASSHE) Undergraduate Research Conference in Science, Technology, Engineering and Mathematics (STEM) at Millersville University in Millersville (PA, USA), Oct. 27

Munoz*, Salazar* & Sanders (2018) Characterization of Cytochrome c Heme Lyase Mutants involved in Binding and Ligation of Heme and Apocytochrome c. 29th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 20

Nunez* & Sanders (2017) Insertion of a Heme Binding Motif at five different Sites of Green Fluorescence Protein. 4thAnnual Pennsylvania State System of Higher Education (PASSHE) Undergraduate Research Conference in Science, Technology, Engineering and Mathematics (STEM) at Millersville University in Millersville (PA, USA), Nov. 4

Nunez* & Sanders (2017) Engineering Green Fluorescent Protein Derivatives with Cytochrome c Heme Binding Sites. 45th Middle Atlantic Regional Meeting (MARM) of the American Chemical Society (ACS) in Hershey (PA, USA), June 4-6

Wan* & Sanders (2017) Plant mitochondrial and chloroplast Cytochrome c Polypeptides as Substrates of an evolutionarily distinct eukaryotic Cytochrome c Biogenesis System. Pennsylvania Academy of Science (PAS) 93rd Annual Meeting, Wilkes-Barre (PA, USA), March 31-April 2

Rimal*, Junker & Sanders (2016) Investigating the Role of a conserved Glutamic Acid in Cytochrome c Heme Lyase for the Biogenesis of Cytochrome c. 252ndAmerican Chemical Society (ACS) National Meeting and Exposition, Philadelphia (PA, USA), August 21-25

Rimal*, Zhang*, Junker & Sanders (2016) The Role of Glutamic Acid E133 in the Catalytic Activity of Yeast Cytochrome c Heme Lyase. Pennsylvania Academy of Science (PAS) 92nd Annual Meeting, Doylestown (PA, USA), April 1-3

Kulick*, Junker & Sanders (2015) Investigating the Catalytic Mechanism of Yeast Cytochrome c Heme Lyase through Mutation of highly conserved Amino Acid Residues. American Society for Biochemistry and Molecular Biology (ASBMB) Annual Meeting in Boston (MA, USA), March 28-April 1 (The FASEB Journal 29:1_supplement)

Greenawalt*, Junker & Sanders (2014) Understanding the Substrate Specificity of Cytochrome c Heme Lyase. 25th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 12

Kulick*, Rimal*, Junker & Sanders (2014) Amino Acid Substitutions in Yeast Cytochrome c Heme Lyase Protein and resulting Effects on Heme and Apoprotein Binding Mechanism. 25th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 12

Mato*, Drexinger*, Junker & Sanders (2014) Testing the Evolutionary Conservation of Proteins mediating Cytochrome c Biogenesis in Yeast and Humans. 25th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 12

Greenawalt*, Junker & Sanders (2014) Testing the Ability of Cytochrome c Heme Lyase to attach nonnative plant mitochondrial and chloroplast c-type Cytochromes with Heme Cofactors. Thomas Jefferson University Chapter of Sigma Xi Student Research Day, Philadelphia (PA, USA), April 2

Kulick*, Junker & Sanders (2014) Mutational Studies on the Heme and Apoprotein Binding Mechanism of Yeast Cytochrome c Heme Lyase. Thomas Jefferson University Chapter of Sigma Xi Student Research Day, Philadelphia (PA, USA), April 2

Blakely*, Kostomiris*, Mato*, Kulick*, Junker & Sanders (2013) Uncovering Amino Acid Residues critical for Binding and Catalysis of Heme and Apoprotein Substrates by Yeast Cytochrome c Heme Lyase. 1st Pennsylvania State System of Higher Education (PASSHE) Conference in Science, Technology, Engineering, and Mathematics (STEM) at Slippery Rock University in Slippery Rock (PA), Nov. 15 and 16

Greenawalt*, Andreyko*, Junker & Sanders (2013) Compatibility of plant chloroplast and mitochondrial Cytochromes c with a nonnative eukaryotic Cytochrome c Biogenesis System. 1st Pennsylvania State System of Higher Education (PASSHE) Conference in Science, Technology, Engineering, and Mathematics (STEM) at Slippery Rock University in Slippery Rock (PA), Nov. 15 and 16

Drexinger*, Ulas*, Junker & Sanders (2013) Construction of a Plasmid Vector System using a fluorescent Selection Marker for Cloning and Coexpressing tag-fused Genes of Interest. 1st Pennsylvania State System of Higher Education (PASSHE) Conference in Science, Technology, Engineering, and Mathematics (STEM) at Slippery Rock University in Slippery Rock (PA), November 15 and 16

Blakely*, Kostomiris*, Junker & Sanders (2013) The molecular Mechanism of Heme Attachment by Cytochrome c Heme Lyase. 24th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 19

Dougherty*, Drexinger*, Junker & Sanders (2013) Engineering a GFP-based Screening Method for tag-fused Gene of Interest Cloning and Coexpression Vectors. 24th Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 19

Blakely*, Kostomiris*, Junker & Sanders (2013) Characterization of Mutations in the putative Heme b Binding Site of Yeast Cytochrome c Heme Lyase. Thomas Jefferson University Chapter of Sigma Xi Student Research Day, Philadelphia (PA, USA), April 17

Fredericks*, Weller*, Hoppes*, Sanders & Junker (2012) Biochemical Basis for Apoptosis Regulation by human Holocytochrome c Synthetase. 244thAmerican Chemical Society (ACS) National Meeting and Exposition, Philadelphia (PA, USA), August 19-23

Fredericks*, Weller*, Hoppes*, Blakely*, Junker & Sanders (2012) Developing an in vitro System for Determining the Biochemical Mechanism of Apoptosis Regulation by human Holocytochrome c Synthetase. Pennsylvania Academy of Science (PAS) 88th Annual Meeting, Allentown (PA, USA), March 30-April 1

Fredericks*, Weller*, Chwiecko*, Sanders & Junker (2011) Testing the biochemical Mechanism for the Activation of Apoptosis by the mitochondrial Enzyme Cytochrome c Heme Lyase. 42nd Middle Atlantic Regional Meeting (MARM) of the American Chemical Society (ACS) in College Park (MD, USA), May 21-24

Chwiecko*, Fredericks*, Weller*, Junker & Sanders (2011) Induction of programmed Cell Death by Cytochrome c Heme Lyase via Association with the Inhibitor of Apoptosis Protein. 22nd Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 8

Verissimo, Honghui, Sanders & Daldal (2011) CcmI is an Apocytochrome Chaperone during Cytochrome c Maturation. 28th Eastern Regional Photosynthesis Meeting, Woods Hole (MA, USA), April 1-3

Chwiecko*, Fredericks*, Weller*, Junker & Sanders (2011) Novel Activation Mechanism of Programmed Cell Death via Interaction of Cytochrome c Heme Lyase with the Inhibitor of Apoptosis Protein. Pennsylvania Academy of Science (PAS) 87th Annual Meeting, Altoona (PA, USA), April 1-3

Desir*, Chwiecko*, Jones*, Junker & Sanders (2010) Investigating the proapoptotic Activity of Cytochrome c Heme Lyase via Interaction with the Inhibitor of Apoptosis Protein. 21st Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 23

Geisler*, Fredericks* & Sanders (2010) Design of a Screening Method for heterologous Cytochrome c Biogenesis in Escherichia coli. 21st Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 23

Kauffman* & Sanders (2010) The catalytic Mechanism of Cytochrome c Heme Lyase from Saccharomyces cerevisiae – Analysis of Mutations in the proposed CPV Heme Binding Motifs. 21st Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 23

Shelly*, Karetsky* & Sanders (2010) Living in a Biopond – Isolation and Analysis of anaerobic phototrophic Bacteria. 21st Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 23

Weller*, Himeback*, Grapsy*, Flaherty* & Sanders (2010) Microbes found in Skim Milk Sources. 21st Annual Saint Joseph’s University Sigma Xi Student Research Symposium, Philadelphia (PA, USA), April 23

Jones*, Chwiecko*, Desir*, Junker & Sanders (2010) The Effect of Cytochrome c Heme Lyase on Activation of programmed Cell Death via Binding to the Inhibitor of Apoptosis Protein. Commonwealth of Pennsylvania University Biologists (CPUB) 41st Annual Meeting, East Stroudsburg (PA, USA), April 16-18

Shelly*, Karetsky* & Sanders (2010) Isolation and Characterization of anaerobic phototrophic Bacteria. Commonwealth of Pennsylvania University Biologists (CPUB) 41st Annual Meeting, East Stroudsburg (PA, USA), April 16-18

Geisler*, Fredericks* & Sanders (2010) Development of a phenotypical Selection System for heterologous Holocytochrome c Production in Escherichia coli. Commonwealth of Pennsylvania University Biologists (CPUB) 41st Annual Meeting, East Stroudsburg (PA, USA), April 16-18

Kauffman* & Sanders (2010) Effect of Mutations in the proposed CPV Heme Binding Motifs of Cytochrome c Heme Lyase on its catalytic Activity. Commonwealth of Pennsylvania University Biologists (CPUB) 41st Annual Meeting, East Stroudsburg (PA, USA), April 16-18

Flaherty*, Grapsy*, Himeback*, Weller* & Sanders (2010) What’s in your milk? – Characterization of unknown Organisms from Skim Milk Sources. Commonwealth of Pennsylvania University Biologists (CPUB) 41st Annual Meeting, East Stroudsburg (PA, USA), April 16-18

Jones*, Desir*, Foster*, Chwiecko*, Junker & Sanders (2010) Probing Cytochrome c Heme lyase binding to the Inhibitor of Apoptosis Protein as a new Mechanism for Activating Apoptosis (Programmed Cell Death). Pennsylvania Academy of Science (PAS) 86th Annual Meeting, Camp Hill (PA, USA), April 9-11

Onder, Sanders, Turkarslan & Daldal (2009) Redox-linked Production of Energy Transduction Components in the phototrophic Bacterium Rhodobacter capsulatus. Department of Energy (DOE)-Basic Energy Sciences (BES) Photosynthetic Systems Research Meeting, Annapolis (MD, USA), October 25-28

Onder, Turkarslan, Sanders & Daldal (2009) Cytochrome c Maturation, Disulfide Bond Formation and periplasmic Protein Degradation in Rhodobacter capsulatus. 13th International Symposium on Phototrophic Prokaryotes, Montreal (Canada), August 9-14

(Kutztown University Undergraduate Students are indicated with *)

 

Last updated by C. Sanders on 06/16/2019